Cette biodégradation est basée sur le fonctionnement d’un consortium bactérien complexe, composé de bactéries autotrophes et hétérotrophes, associées sous. Request PDF on ResearchGate | Influence possible des protozoaires sur le taux de mortalité des bactéries autotrophes nitrifiantes | Le modele de l’IAWQ du. Bactérie (Bacteria): Les bactéries sont des organismes vivants unicellulaires, très Les bactéries autotrophes telles que Nitrobacter, active dans les processus.

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They consist in measuring differences of ammonium, nitrite and nitrate dynamics in an inhibited and control sample during time. Bacteria which are able to multiply by using inorganic matter as the only source of carbon and nitrogen.

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A good alternative to these methods consists in the measurement of potential nitrifying activity which is correlated to the nitrifying biomass.

This factors allowed us to establish a relationship between potential nitrifying activity measurements and nitrifying biomass: The most frequently used enumeration methods of nitrifying le are not very satisfactory. The measurement of the activity and biomass of nitrifying bacteria is thus essential to understand and quantify the general nitrogen fluxes in those environments.

Different methods of measuring the nitrifying activity exist.

Term Bank – bact – French English Dictionary

To measure potential nitrifying activities, the optimal growth conditions of nitrifying bacteria were determined on enrichment cultures: By acting this way, inhibition of autotrophic C incorporation bactrie nitrifying bacteria was also complete, while heterotrophic incorporation was unaffected.

The language you choose must correspond to the language of the lee you have entered. Pure cultures of Nitrosomonas europaea and Nitrobacter winogradskyi were obtained from the National Collection of Industrial and Marine Bacteria Aberdeen, Scotland and a continuous enrichment culture of mixed heterotrophic bacteria, without nitrifying organisms, was obtained with a freshwater inoculum by imposing a residence time of 2 hours less than the generation time of nitrifying bacteria.


Environmental Studies and Analyses [1]. Revue des sciences de l’eau 11, no.

This potential activity can be used to estimate the nitrifying biomass by considering a conversion factor of 0. Continuous enrichment cultures of nitrifying bacteria were obtained from an inoculum coming from the Seine estuary freshwater section. Nitrifying activity, nitrifying biomass, 14C-bicarbonate incorporation, nitrification inhibitors, N-serve, chlorate.

The method can be used in in situ conditions, to allow real nitrifying activities measurements in samples. C incorporation rates are measured by the incubation of samples with 14C-bicarbonate, the samples being filtered on 0. Classical culture techniques most probable number and immunofluorescence techniques are known to greatly underestimate the numbers of active organisms.

The organic solvent enhanced heterotrophic incorporation of C which totally masked out the autotrophic contribution of nitrifying bacteria.

These methods can be applied as long as the inhibitors are specific for nitrifying bacteria, and activities are high enough to allow the measurement of concentration variations during incubation times which are not too long. The first methods developed were based on the use of specific nitrification inhibitors: The main differences of bacferie protocol to the original ones is that we propose the use of a combination of 2 inhibitors, N-serve and chlorate, and the elimination by evaporation of the organic solvent of N-serve ethanol to avoid any interference with the heterotrophic populations.

Our conclusion is that the results presented in this paper allow the validation of the 14C-bicarbonate incorporation method with and without inhibitors to measure the nitrifying activity.


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Revue des sciences de l’eau11 2— For this reason N-serve was added in the empty flask before the sample to allow the evaporation of the solvent. In which subject field? baxterie

Biovolumes were estimated according to cell size and converted in biomasses according to a conversion factor determined experimentally with a carbon analyser. In this case, carbon incorporation rates can be converted in ammonium oxidation rates with the use of the conversion factor 0.

However, the ethanolic solution of N-serve had an unwanted result on C incorporation. Several methods were used for our study: Ammonium was measured with the indophenol blue method, nitrate was reduced in nitrite on a cadmium bed and nitrite was measured with the sulfanilamide method.

The inhibitors effects were also determined on the C incorporation rates of heterotrophic bacteria. At the present time, the most used methods are dealing with isotopic tracers: A collection of writing tools that cover the many facets of English and French grammar, style and usage.

Download the article in PDF to read it. Writing tools A collection of writing tools that cover the many facets of English and French bqcterie, style and usage.